How to Read a Chromatogram?

Over the years chromatography has gained an enviable position in analytical laboratories involving separation and quantification of organic compound mixtures. However, a chromatogram is not a display of results in concentration units but rather a graphical display in real time of peaks generated as the separated components pass through the detector. The chromatogram makes little sense to the layman as the peaks provide no information on the identity of the mixture components nor any information on the amount present.

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